Refine your search
Collections
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Heru Prasetyo, R.
- Immunoglobulin Subclass in Experimental Murine Toxocara cati Infection
Abstract Views :143 |
PDF Views:0
Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.
Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.
Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.
Authors
Affiliations
1 Department of Parasitology, Universitas Airlangga, Surabaya, East Java, ID
1 Department of Parasitology, Universitas Airlangga, Surabaya, East Java, ID
Source
Veterinary World, Vol 10, No 11 (2017), Pagination: 1333-1336Abstract
Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.
Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.
Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.
Keywords
Diagnostic Kit, Enzyme-Linked Immunosorbent Assay, Human, Immunoglobulin G Subclass, Monoclonal Antibody, Toxocariasis, Toxocara cati.Full Text
- Honey Can Repairing Damage of Liver Tissue Due to Protein Energy Malnutrition Through Induction of Endogenous Stem Cells
Abstract Views :107 |
PDF Views:0
Materials and Methods: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique.
Results: There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45.
Conclusion: Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue.
Authors
Affiliations
1 Department of Parasitology, Universitas Airlangga, Surabaya, East Java, ID
2 Department of Veterinary Anatomy, Universitas Airlangga, Surabaya, East Java, ID
1 Department of Parasitology, Universitas Airlangga, Surabaya, East Java, ID
2 Department of Veterinary Anatomy, Universitas Airlangga, Surabaya, East Java, ID
Source
Veterinary World, Vol 10, No 6 (2017), Pagination: 711-715Abstract
Aim: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells.Materials and Methods: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique.
Results: There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45.
Conclusion: Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue.
Keywords
Endogenous Stem Cells, Honey, Liver Tissue, Protein Energy Malnutrition, Regeneration.Full Text